Prepared microscope slide: Hamster Spleen Set of 2
Both views of these slides are at 40x. These are both fixed in Bouin’s fluid They are then stained with either Harris’ Hematoxylin or Heidenhain’s Hematoxylin. Then they are counter-stained. Samples are 10μ thick. The main slide shown is stained with Harris’ Hematoxylin and counter-stained with Methyl green. There is 1 additional slide in the set. It is stained with Heidenhain’s Hematoxylin and counter-stained with Methyl green. It may be seen by clicking either of the following line.
Click here for a view of Hamster Spleen fixed in Bouin’s Fluid and stained with Heidenhain’s Hematoxylin and counter-stained with Methyl green, at 40x.
These slides were prepared sometime prior to the 1950s by an individual named Kirschbaum.
Bouin’s fluid contains picric acid, which has the property that it renders tissue more readily stained by acid dyes. Harris’ Hematoxylin is a nuclear stain that produces a deep purple blue. Heidenhain’s Hematoxylin uses two solutions, the first being an iron mordant such as ferric chloride or ferrous ammonium sulfate followed by an application of hematoxylin.
Forensics Lab Supply Number 3862712.
Click here to purchase Eosin Y stain.
Click here to purchase Methyl green stain.
Click here to purchase Orange G stain.
Click here to purchase Hematoxylin stain.
To view archives of Prepared Pathology Slides, CLICK HERE.
To view archives of other Historic Prepared Slides, CLICK HERE.
To view archives of other Prepared Slides made by Lee Gonzales, CLICK HERE.
These slides is placed here primarily so that everyone with an interest would be able to view them as though viewing through a microscope. For additional information, contact me at staff @ grauhall.com.
Image copyright held by Alan J. Pedersen.
Tags: prepared microscope slide
Not made in China.