Prepared microscope slide: Hamster Sperm Smear Individual slide
This slide is shown at 100x with an additional view at 400x. It is fixed in Bouin’s fluid then stained with Harris’ Hematoxylin, then counter-stained with Eosin. The second view may be seen by clicking the following line.
Click here for a view of Hamster Sperm Smear fixed in Bouin’s Fluid and stained with Harris’ Hematoxylin and counter-stained with Eosin, at 400x.
These slides were prepared sometime prior to the 1950s by an individual named Kirschbaum.
Bouin’s fluid contains picric acid, which has the property that it renders tissue more readily stained by acid dyes. Harris’ Hematoxylin is a nuclear stain that produces a deep purple blue. Heidenhain’s Hematoxylin uses two solutions, the first being an iron mordant such as ferric chloride or ferrous ammonium sulfate followed by an application of hematoxylin.
Forensics Lab Supply Number 3862711.
Click here to purchase Eosin Y stain.
Click here to purchase Methyl green stain.
Click here to purchase Orange G stain.
Click here to purchase Hematoxylin stain.
To view archives of Prepared Pathology Slides, CLICK HERE.
To view archives of other Historic Prepared Slides, CLICK HERE.
To view archives of other Prepared Slides made by Lee Gonzales, CLICK HERE.
These slides is placed here primarily so that everyone with an interest would be able to view them as though viewing through a microscope. For additional information, contact me at staff @ grauhall.com.
Image copyright held by Alan J. Pedersen.
Tags: prepared microscope slide
Not made in China.